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Time-lapse Imaging of Primary Preneoplastic Mammary Epithelial Cells Derived from Genetically Engineered Mouse Models of Breast Cancer

Article DOI: 10.3791/50198-v • 11:47 min • February 8th, 2013
February 8th, 2013 •
1, 1, 1, 1,2, 1,2, 3, 3, 1,2,4,5
1Department of Oncology, Georgetown University, 2Lombardi Comprehensive Cancer Center, Georgetown University, 3Stem Cell Dynamics, Helmholtz Zentrum München - German Research Center for Environmental Health, 4Department of Medicine, Georgetown University, 5Department of Nanobiomedical Science and WCU Research Center of Nanobiomedical Science, Dankook University

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Time-lapse imaging is used to assess behavior of primary preneoplastic mammary epithelial cells derived from genetically engineered mouse models of breast cancer risk to determine if there are correlations between specific behavioral parameters and distinct genetic lesions.

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Time-lapse Imaging Primary Preneoplastic Mammary Epithelial Cells Genetically Engineered Mouse Models Breast Cancer Cell Behavior Cell Lifetimes Apoptotic Cell Numbers Morphological Changes Colony Formation Genetic Lesions Mammary Gland Resection Single-cell Suspensions Live-cell Imaging Microscope Incubator Chamber Well Plate Cellular Behaviors Cell Colony Formation Cell Proliferation Apoptosis Morphological Changes Single-cell Tracking
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