Monitoring Dendritic Cell Migration using <sup>19</sup>F / <sup>1</sup>H Magnetic Resonance Imaging

Helmar Waiczies; Martin Guenther; Julia Skodowski; Stefano Lepore; Andreas Pohlmann; Thoralf Niendorf; Sonia Waiczies

doi:10.3791/50251

Monitoramento da Migração de Células dendríticas usando 19 F / 1 H Ressonân...

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Immunology and Infection

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JoVE Journal Immunology and Infection

Monitoring Dendritic Cell Migration using ¹⁹F / ¹H Magnetic Resonance Imaging

Monitoramento da Migração de Células dendríticas usando 19 F / 1 H Ressonância Magnética

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08:12 min

March 20, 2013

DOI: 10.3791/50251-v

Helmar Waiczies^1,2, Martin Guenther^1,2, Julia Skodowski^1,2, Stefano Lepore^1,2, Andreas Pohlmann², Thoralf Niendorf^1,2, Sonia Waiczies^1,2

¹Experimental and Clinical Research Center,A joint cooperation between the Charité Medical Faculty and the Max Delbrück Center for Molecular Medicine, ²Berlin Ultrahigh Field Facility (B.U.F.F.),Max Delbrück Center for Molecular Medicine

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Overview

This protocol outlines a method for tracking the migration of dendritic cells using non-invasive fluorine MRI in combination with proton MRI. The process involves labeling murine bone marrow-derived dendritic cells with fluorine-rich particles and monitoring their movement to the draining lymph nodes.

Key Study Components

Area of Science

Neuroscience
Imaging Techniques
Cell Tracking

Background

Dendritic cells play a crucial role in the immune response.
Tracking cell migration is essential for understanding immune functions.
Traditional tracking methods may have limitations.
Fluorine MRI offers a non-invasive alternative for monitoring cell movement.

Purpose of Study

To visualize the migration of dendritic cells to lymph nodes.
To utilize fluorine MRI for tracking labeled cells.
To compare the effectiveness of this method with traditional techniques.

Methods Used

Preparation of murine bone marrow-derived dendritic cells.
Labeling of cells with fluorine-rich particles.
Administration of labeled cells into the hind limbs of mice.
Imaging the migration of cells using fluorine and proton MRI.

Main Results

Successful visualization of dendritic cell migration to lymph nodes.
Demonstrated the efficacy of fluorine MRI in tracking cells.
Highlighted the advantages over traditional iron oxide nanoparticle methods.
Provided insights into the dynamics of immune cell movement.

Conclusions

Fluorine MRI is a powerful tool for non-invasive cell tracking.
This method enhances understanding of immune responses.
Further research could expand applications in immunology.

Frequently Asked Questions

What is the significance of tracking dendritic cells?

Tracking dendritic cells is crucial for understanding their role in the immune response and how they migrate to lymph nodes.

How does fluorine MRI differ from traditional imaging methods?

Fluorine MRI allows for non-invasive tracking of cells without the complications associated with iron oxide nanoparticles.

What are the advantages of using murine models in this study?

Murine models provide a controlled environment to study immune responses and cell migration effectively.

Can this method be applied to other cell types?

Yes, the fluorine labeling technique can potentially be adapted for tracking various cell types in different contexts.

What future applications could arise from this research?

Future applications may include enhanced immunotherapy strategies and better understanding of autoimmune diseases.

Is fluorine MRI widely used in current research?

Fluorine MRI is gaining attention but is still being explored for broader applications in research.

Rastreamento de células usando MRI ganhou notável atenção nos últimos anos. Este protocolo descreve a marcação das células dendríticas com flúor (

O objetivo geral do experimento a seguir é visualizar a migração de células dendríticas para o linfonodo de drenagem usando ressonância magnética de flúor não invasiva ou ressonância magnética em combinação com ressonância magnética de prótons convencional. Isso é conseguido preparando primeiro as células dendríticas derivadas da medula óssea murina e marcando as células totalmente diferenciadas com partículas ricas em flúor. Durante a segunda etapa, as células marcadas com flúor são administradas intracutâneas nos membros posteriores de um camundongo, garantindo a transmigração eficiente das células administradas.

Em seguida, a região anatômica de interesse é visualizada por flúor, ressonância magnética de prótons, para monitorar a migração das células dendríticas da pele para os linfonodos de drenagem. Em última análise, a migração de células dendríticas da área de aplicação para o linfonodo de drenagem pode ser visualizada por ressonância magnética com flúor. A principal vantagem deste método sobre outras técnicas de rastreamento celular, como aquelas que requerem nanopartículas de óxido de ferro, é que o piso ligado ao carbono está praticamente ausente em todos os organismos vivos.

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