Waiting
Processando Login

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Bioengineering / Creating Adhesive and Soluble Gradients for…
JoVE Journal
Bioengineering

É necessário ter uma assinatura JoVE para assistir este

JoVE Journal Bioengineering
Creating Adhesive and Soluble Gradients for Imaging Cell Migration with Fluorescence Microscopy
Routine Maintenance of Micro-Device System to Promote Optimum Coral Growth
Journal (Bioengineering)
Routine Maintenance of Micro-Device System to Promote Optimum Coral Growth
An Experimental and Finite Element Protocol to Investigate the Transport of Neutral and Charged Solutes across Articular Cartilage
Journal (Bioengineering)
An Experimental and Finite Element Protocol to Investigate the Transport of Neutral and Charged Solutes across Articular Cartilage
 

Creating Adhesive and Soluble Gradients for Imaging Cell Migration with Fluorescence Microscopy

Article DOI: 10.3791/50310-v • 13:10 min • April 4th, 2013
April 4th, 2013 •
1, 1, 2, 1, 1, 2, 1, 1
1Centre for Vascular Research and Australian Centre for Nanomedicine, The University of New South Wales, 2School of Chemistry and Australian Centre for Nanomedicine, The University of New South Wales

Capítulos

Resumo
Automatic Translation

A method for the assembly of adhesive and soluble gradients in a microscopy chamber for live cell migration studies is described. The engineered environment combines antifouling surfaces and adhesive tracks with solution gradients and therefore allows one to determine the relative importance of guidance cues.

Tags

Adhesive Gradients Soluble Gradients Cell Migration Fluorescence Microscopy Microfluidic Chamber Live Cell Imaging Copolymer Poly-L-lysine Polyethylene Glycol Passivation Non-specific Adsorption Biomolecules Cells Microcontact Printing Dip Pen Lithography Streptavidin Biotinylated Peptide Arginine-glycine-aspartic Acid (RGD) Microfluidic Device Chemoattractant Fetal Bovine Serum (FBS)
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter