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RNAi-mediated Double Gene Knockdown and Gustatory Perception Measurement in Honey Bees (Apis mellifera)
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RNAi-mediated Double Gene Knockdown and Gustatory Perception Measurement in Honey Bees (Apis mellifera)

Article DOI: 10.3791/50446-v • 10:57 min • July 25th, 2013
July 25th, 2013 •
1, 1, 1,2
1School of Life Sciences, Arizona State University, 2Department of Chemistry, Biotechnology, and Food Science, Norwegian University of Life Sciences

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In this protocol, we describe two strategies that simultaneously suppress two genes (double gene knockdown) in honey bees. Then we present how to use the proboscis extension response (PER) assay to study the effect of double gene knockdown on honey bee gustatory perception.

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RNAi-mediated Double Gene Knockdown Gustatory Perception Measurement Honey Bees Apis Mellifera RNA Interference Double-stranded RNA Injections Proboscis Extension Response Assay Gene Manipulation Gene Knockdown Techniques Vitellogenin Ultraspiracle Regulatory Feedback Loop Interrelationships Between Genes Insect Species Behavioral Maturation Age At Onset
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