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Metabole Labeling en Membrane Fractionation for Comparative Proteomic analyse van
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Metabole Labeling en Membrane Fractionation for Comparative Proteomic analyse van

Article DOI: 10.3791/50535-v • 11:44 min • September 28th, 2013
September 28th, 2013 •
1, 1, 1,2
1Signaling Proteomics, Max Plank Institute of Molecular Plant Physiology, 2Department of Plant Systems Biology, University of Hohenheim

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Hier beschrijven we een robuuste werkwijze voor de fractionering van plantaardige plasmamembranen in reinigingsmiddelenbestendige en detergent oplosbare membranen gebaseerd op een mengsel van gelabelde en in vivo volledig 15N gemerkt Arabidopsis thaliana celculturen. De procedure wordt toegepast voor vergelijkende proteomics studies om signalering processen te begrijpen.

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Lege waarde Structuur cellulaire planten genetisch gemodificeerde Arabidopsis membraanlipiden intracellulaire signalering peptiden en eiwitten membraaneiwitten isotooplabelen Proteomics planten Arabidopsis thaliana metabole etikettering stabiele isotopen schorsing celculturen plasma membraan fractionering twee fasen systeem reinigingsmiddelenbestendige membranen (DRM) massaspectrometrie membraan microdomeinen kwantitatieve proteomics
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