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Isolation of Sensory Neurons of Aplysia californica for Patch Clamp Recordings of Glutamatergic Currents

Article DOI: 10.3791/50543-v • 09:40 min • July 10th, 2013
July 10th, 2013 •
1, 1, 1, 1, 1
1Division of Marine Biology and Fisheries, Rosenstiel School of Marine and Atmospheric Sciences, University of Miami

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We describe the dissection of the nervous system of the marine sea hare Aplysia after anesthesia, the isolation of neurons for short term-tissue culture, and recordings of single cell ion currents via the patch clamp technique.

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Isolation Sensory Neurons Aplysia Californica Patch Clamp Recordings Glutamatergic Currents Nervous System Function Learning And Memory Minimally Dissected Animal Neuronal Co-culture Isolated Neuronal Preparation Dissociated Cells Short Term Culture Biophysical Characterization Ion Currents Patch Clamp Techniques Targeted Modulation Protocol For Preparing Cultures Glutamatergic Sensory Neurons Pleural Ganglia Buccal Ganglia
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