Transplantation of Olfactory Ensheathing Cells to Evaluate Functional Recovery after Peripheral Nerve Injury

Nicolas Guerout; Alexandre Paviot; Nicolas Bon-Mardion; Axel Honor&#233;; Rais OBongo; C&#233;lia Duclos; Jean-Paul Marie

doi:10.3791/50590

Transplante de células olfativas Ensheathing para avaliar a recuperação funcional após a lesão do...

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Neuroscience

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JoVE Journal Neuroscience

Transplantation of Olfactory Ensheathing Cells to Evaluate Functional Recovery after Peripheral Nerve Injury

Transplante de células olfativas Ensheathing para avaliar a recuperação funcional após a lesão do nervo periférico

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10:33 min

February 23, 2014

DOI: 10.3791/50590-v

Nicolas Guerout^1,2, Alexandre Paviot^1,3, Nicolas Bon-Mardion^1,3, Axel Honoré¹, Rais OBongo^1,4, Célia Duclos¹, Jean-Paul Marie^1,3

¹UPRES EA3830, Institute for Research and Innovation in Biomedicine,University of Rouen, ²Neuroscience,Karolinska Institutet, ³Otorhinolaryngology, Head and Neck Surgery Department,Rouen University Hospital, ⁴Otorhinolaryngology, Head and Neck Surgery Department,Amiens University Hospital

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Overview

This study presents a model of cellular transplantation using olfactory ensheathing cells (OECs) in a laryngeal nerve injury model. OECs, derived from neural crest cells, facilitate the growth of primary olfactory neurons, making them suitable for transplantation.

Key Study Components

Area of Science

Neuroscience
Cellular transplantation
Regenerative medicine

Background

Olfactory ensheathing cells (OECs) support the regeneration of olfactory neurons.
OECs can be harvested from olfactory mucosa or bulbs.
Recurrent laryngeal nerve injuries can impact vocal cord function.
Cellular transplantation may enhance nerve regeneration and functional recovery.

Purpose of Study

To evaluate the effectiveness of OECs in promoting axonal regrowth.
To assess functional recovery of the vocal cords post-transplantation.
To establish a reliable model for future studies on nerve injury and repair.

Methods Used

Sectioning and anastomosis of the recurrent laryngeal nerve.
Transplantation of OECs obtained from olfactory sources.
Evaluation of recovery through laryngoscopy and electromyography.
Histological studies of the recurrent laryngeal nerve.

Main Results

OECs improved axonal regrowth of the recurrent laryngeal nerve.
Functional recovery of vocal cords was observed through laryngoscopy.
Electromyography confirmed nerve function restoration.
Histological analysis supported the regenerative effects of OECs.

Conclusions

Olfactory ensheathing cells are effective in nerve regeneration.
Transplantation of OECs can lead to functional recovery in laryngeal nerve injuries.
This model can be utilized for further research in nerve repair strategies.

Frequently Asked Questions

What are olfactory ensheathing cells?

Olfactory ensheathing cells are specialized cells that support the growth of olfactory neurons and are derived from neural crest cells.

How are OECs obtained for transplantation?

OECs can be harvested from the olfactory mucosa or olfactory bulbs.

What is the significance of the recurrent laryngeal nerve?

The recurrent laryngeal nerve is crucial for vocal cord function, and injuries to this nerve can lead to voice impairment.

What methods are used to evaluate recovery after OEC transplantation?

Recovery is evaluated through laryngoscopy, electromyography, and histological studies.

What were the main findings of this study?

The study found that OECs significantly improved axonal regrowth and functional recovery of the recurrent laryngeal nerve.

Can this model be used for future research?

Yes, this model provides a basis for further studies on nerve injury and repair.

Células olfativas ensheathing (OECO) são células da crista neural que permitem o crescimento dos neurônios olfativos primários. Esta propriedade específica pode ser utilizado para transplantação celular. Apresentamos aqui um modelo de transplante celular baseado na utilização de OECs em um modelo de lesão do nervo laríngeo.

O objetivo geral deste procedimento é apresentar um modelo de transplante celular baseado no uso de células olfativas e revestidas em um modelo de lesão do nervo laríngeo. Isso é realizado primeiro seccionando e realizando a anastomose do nervo laríngeo recorrente. Em seguida, as células olfativas e de revestimento obtidas da mucosa olfatória ou bulbos olfatórios são transplantadas.

Então, dois a três meses após a cirurgia, os animais são avaliados por laringoscopia e eletromiografia. Finalmente, o nervo laríngeo recorrente é isolado para estudos histológicos. Em última análise, podem ser obtidos resultados que mostram células olfativas e de revestimento melhorando o crescimento axonal do nervo laríngeo recorrente e a recuperação funcional das cordas vocais por meio de laringoscopia, eletromiografia e medidas histológicas.

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