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Using Click Chemistry to Measure the Effect of Viral Infection on Host-Cell RNA Synthesis

Article DOI: 10.3791/50809-v • 09:35 min • August 9th, 2013
August 9th, 2013 •
1, 1, 1, 1, 1
1Department of Pathology, Sealy Center for Vaccine Development, Center for Biodefense and Emerging Infectious Diseases, University of Texas Medical Branch

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This method describes the use of click chemistry to measure changes in host cell transcription after infection with the Rift Valley fever virus (RVFV) strain MP-12. Results can be visualized qualitatively via fluorescence microscopy or obtained quantitatively through flow cytometry. This method is adaptable for use with other viruses.

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Click Chemistry Viral Infection Host-cell RNA Synthesis Transcriptional Activity Radioactive Nucleosides Autoradiography Scintillation Counting Halogenated Uridine Analogs 5-bromouridine (BrU) Immunostaining Radioactive Isotopes Specialized Equipment Detection Of BrU Click Chemistry Copper-catalyzed Triazole Formation Azide Alkyne Rapid And Highly Sensitive Method Uridine Analog 5-ethynyluridine (EU) Fluorescent Azide Visualization Rift Valley Fever Virus
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