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Identification of a Murine Erythroblast Subpopulation Enriched in Enucleating Events by Multi-spectral Imaging Flow Cytometry
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Identification of a Murine Erythroblast Subpopulation Enriched in Enucleating Events by Multi-spectral Imaging Flow Cytometry

Article DOI: 10.3791/50990-v • 09:40 min • June 6th, 2014
June 6th, 2014 •
1, 1, 1, 2, 1, 1
1Cancer and Blood Diseases Institute, Cincinnati Children's Hospital Medical Center, University of Cincinnati College of Medicine, 2IBM

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The present protocol describes a novel method of identifying a population of enucleating orthochromatic erythroblasts by multi-spectral imaging flow cytometry, providing a visualization of the erythroblast enucleation process.

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Murine Erythroblast Subpopulation Enucleating Events Multi-spectral Imaging Flow Cytometry Erythropoiesis Enucleation Mechanism Key Proteins And Structures Microscopy Sufficient Number Of Cells Analysis Protocol Multiparameter High-speed Cell Imaging Immunofluorescent Microscopy Flow Cytometry Statistical Analysis In Vitro Erythropoiesis Culture Methods Synchronization Of Erythroblasts Capturing Enucleation Staining Of Erythroblasts Fixation And Permeabilization Intracellular Proteins Lipid Rafts Multi-spectral Imaging Flow Cytometry Parameters
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