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Easy Measurement of Diffusion Coefficients of EGFP-tagged Plasma Membrane Proteins Using k-Space Image Correlation Spectroscopy
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Easy Measurement of Diffusion Coefficients of EGFP-tagged Plasma Membrane Proteins Using k-Space Image Correlation Spectroscopy

Article DOI: 10.3791/51074-v • 11:43 min • May 10th, 2014
May 10th, 2014 •
1, 1, 1, 2, 1
1Institute of Molecular Biology and Genetics and Interdisciplinary Nanoscience Center, Aarhus University, 2Departments of Chemistry and Physics, McGill University

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This paper provides a step by step guide to the fluctuation analysis technique k-Space Image Correlation Spectroscopy (kICS) for measuring diffusion coefficients of fluorescently labeled plasma membrane proteins in live mammalian cells.

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Diffusion Coefficients EGFP-tagged Plasma Membrane Proteins K-Space Image Correlation Spectroscopy Lateral Diffusion Compartmentalization Probe Photophysics Time Correlation Function Fluorescence Microscopy Reciprocal Space Circular Averaging Natural Logarithm Transform Linear Fits Image Analysis
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