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Sample Drift Correction Following 4D Confocal Time-lapse Imaging
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Sample Drift Correction Following 4D Confocal Time-lapse Imaging

Article DOI: 10.3791/51086-v • 10:05 min • April 12th, 2014
April 12th, 2014 •
1, 2, 1
1School of Biological Sciences, Monash University, 2Janelia Farm Research Campus, Howard Hughes Medical Institute

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Time-lapse microscopy allows the visualization of developmental processes. Growth or drift of samples during image acquisition reduces the ability to accurately follow and measure cell movements during development. We describe the use of open source image processing software to correct for three dimensional sample drift over time.

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Sample Drift Correction 4D Confocal Time-lapse Imaging Cellular Behaviors Developmental Processes Sample Movements Drift Correction Image Acquisition Cell Tracking Static Structures Sample Drift Correction Plugin Fiji Distribution Of ImageJ LOCI Tools Correct 3D Drift Plugin Confocal Datasets Phase Correlation Time-lapse Experiments
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