Journal
/
Biologia
/
Analyzing Craniofacial Morphogenesis in Zebrafish Using 4D Confocal Microscopy
JoVE Journal
Biologia
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Biologia
Analyzing Craniofacial Morphogenesis in Zebrafish Using 4D Confocal Microscopy

Analyzing Craniofacial Morphogenesis in Zebrafish Using 4D Confocal Microscopy

DOI:
10.3791/51190-v

•

09:16 min

•

January 30, 2014

•

, ,
1Institute for Cell and Molecular Biology,The University of Texas at Austin

Capítulos

  • 00:05Título
  • 01:07Mounting Zebrafish Embryos for Confocal Microscopy
  • 04:15Define Experiment Settings
  • 07:09Results: Craniofacial Defects
  • 08:31Conclusion

Summary

Tadução automática

Tadução automática

Time-lapse confocal imaging is a powerful technique useful for characterizing embryonic development. Here, we describe the methodology and characterize craniofacial morphogenesis in wild-type, as well as pdgfra, smad5, and smo mutant embryos.

Tags

Craniofacial MorphogenesisZebrafish4D Confocal MicroscopyTime-lapse ImagingFluorescent LabelingNeural CrestCraniofacial Development

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

Two-photon axotomy and time-lapse confocal imaging in live zebrafish embryos

Live Imaging of the Zebrafish Embryonic Brain by Confocal Microscopy

Electroporation of Craniofacial Mesenchyme

Fluorescence-based Measurement of Store-operated Calcium Entry in Live Cells: from Cultured Cancer Cell to Skeletal Muscle Fiber

Visualization of Craniofacial Development in the sox10: kaede Transgenic Zebrafish Line Using Time-lapse Confocal Microscopy

Simultaneous pH Measurement in Endocytic and Cytosolic Compartments in Living Cells using Confocal Microscopy

In vivo Clonal Tracking of Hematopoietic Stem and Progenitor Cells Marked by Five Fluorescent Proteins using Confocal and Multiphoton Microscopy

4D Microscopy of Yeast

Visualizing Lymph Node Structure and Cellular Localization using Ex-Vivo Confocal Microscopy

Analyzing the α-Actinin Network in Human iPSC-Derived Cardiomyocytes Using Single Molecule Localization Microscopy

Read Article