Alternative Cultures for Human Pluripotent Stem Cell Production, Maintenance, and Genetic Analysis

Kevin G. Chen; Rebecca S. Hamilton; Pamela G. Robey; Barbara S. Mallon

doi:10.3791/51519

Journal

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Biologia

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Culturas alternativas para pluripotentes estaminais humanas produção de células, Manutenção e Análise Genética

JoVE Journal
Biologia

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JoVE Journal Biologia

Alternative Cultures for Human Pluripotent Stem Cell Production, Maintenance, and Genetic Analysis

Culturas alternativas para pluripotentes estaminais humanas produção de células, Manutenção e Análise Genética

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12,243 Views

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08:27 min

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July 24, 2014

DOI:

10.3791/51519-v

DOI:

10.3791/51519-v

•

08:27 min

July 24, 2014

•

8 Views

Kevin G. Chen¹, Rebecca S. Hamilton¹, Pamela G. Robey², Barbara S. Mallon¹

¹NIH Stem Cell Unit, National Institute of Neurological Disorders and Stroke,National Institutes of Health, ²Craniofacial and Skeletal Diseases Branch, National Institute of Dental and Craniofacial Research,National Institutes of Health

Summary

Here, we present human pluripotent stem cell (hPSC) culture protocols, based on non-colony type monolayer (NCM) growth of dissociated single cells. This new method, utilizing Rho-associated kinase inhibitors or the laminin isoform 521 (LN-521), is suitable for producing large amounts of homogeneous hPSCs, genetic manipulation, and drug discovery.