RNA Catalyst as a Reporter for Screening Drugs against RNA Editing in Trypanosomes

Houtan Moshiri; Vaibhav Mehta; Reza Salavati

doi:10.3791/51712

Journal

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Biologia

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RNA Catalisador como repórter para Triagem Drogas contra edição de RNA em Tripanossomas

JoVE Journal
Biologia

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JoVE Journal Biologia

RNA Catalyst as a Reporter for Screening Drugs against RNA Editing in Trypanosomes

RNA Catalisador como repórter para Triagem Drogas contra edição de RNA em Tripanossomas

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8,390 Views

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09:19 min

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July 22, 2014

DOI:

10.3791/51712-v

DOI:

10.3791/51712-v

•

09:19 min

July 22, 2014

•

3 Views

Houtan Moshiri*^1,2, Vaibhav Mehta*^1,2, Reza Salavati^1,2,3

¹Department of Biochemistry,McGill University, ²Institute of Parasitology,McGill University, ³McGill Centre for Bioinformatics,McGill University

Summary

A highly sensitive ribozyme-based assay, applicable to high-throughput screening of chemicals targeting the unique process of RNA editing in trypanosomatid pathogens, is described in this paper. Inhibitors can be used as tools for hypothesis-driven analysis of the RNA editing process and ultimately as therapeutics.