Organotypic Slice Cultures to Study Oligodendrocyte Dynamics and Myelination

Robert A. Hill; Jelena Medved; Kiran D. Patel; Akiko Nishiyama

doi:10.3791/51835

Journal

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Neurociência

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Culturas Organotípicas fatia para Estudar Oligodendrocyte Dynamics e Myelination

Organotypic Slice Cultures to Study Oligodendrocyte Dynamics and Myelination

JoVE Journal
Neurociência

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JoVE Journal Neurociência

Organotypic Slice Cultures to Study Oligodendrocyte Dynamics and Myelination

Culturas Organotípicas fatia para Estudar Oligodendrocyte Dynamics e Myelination

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18,246 Views

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09:45 min

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August 25, 2014

DOI:

10.3791/51835-v

DOI:

10.3791/51835-v

•

09:45 min

August 25, 2014

•

12 Views

Robert A. Hill^1,3, Jelena Medved¹, Kiran D. Patel¹, Akiko Nishiyama^1,2

¹Department of Physiology and Neurobiology,University of Connecticut, ²Stem Cell Institute,University of Connecticut, ³Department of Neurology,Yale University School of Medicine

Summary

A technique to study NG2 cells and oligodendrocytes using a slice culture system of the forebrain and cerebellum is described. This method allows examination of the dynamics of proliferation and differentiation of cells within the oligodendrocyte lineage where the extracellular environment can be easily manipulated while maintaining tissue cytoarchitecture.