Improved In-gel Reductive &#946;-Elimination for Comprehensive O-linked and Sulfo-glycomics by Mass Spectrometry

David B. Nix; Tadahiro Kumagai; Toshihiko Katoh; Michael Tiemeyer; Kazuhiro Aoki

doi:10.3791/51840

Journal

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Química

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Melhorou em-gel redutivas β-eliminação para Comprehensive O-ligados e sulfo-glycomics por Espectrometria de Massa

Improved In-gel Reductive β-Elimination for Comprehensive O-linked and Sulfo-glycomics by Mass Spectrometry

JoVE Journal
Química

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JoVE Journal Química

Improved In-gel Reductive β-Elimination for Comprehensive O-linked and Sulfo-glycomics by Mass Spectrometry

Melhorou em-gel redutivas β-eliminação para Comprehensive O-ligados e sulfo-glycomics por Espectrometria de Massa

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11,764 Views

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13:06 min

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November 20, 2014

DOI:

10.3791/51840-v

DOI:

10.3791/51840-v

•

13:06 min

November 20, 2014

•

3 Views

David B. Nix^1,2, Tadahiro Kumagai¹, Toshihiko Katoh^1,3, Michael Tiemeyer^1,2, Kazuhiro Aoki¹

¹Complex Carbohydrate Research Center,University of Georgia, ²Department of Biochemistry and Molecular Biology,University of Georgia, ³Research Institute for Bioresources and Biotechnology,Ishikawa Prefectural University

Transcrição

Summary

In order to comprehensively explore the diversity of O-linked glycans, a new procedure for in-gel reductive β-elimination, combined with permethylation and a rapid phase-partition method, is applied to the analysis of O-linked glycans directly released from glycoproteins resolved by SDS-PAGE and amenable to subsequent glycomic analysis by mass spectrometry.