Usando a sobreposição de ensaio para medir qualitativamente produção bacteriana e sensibilidade para pneumocócica Bacteriocinas
13,566 Views
•
06:05 min
•
September 30, 2014
DOI:
Summary
Automatically generated
Competition in Streptococcus pneumoniae is mediated by bacteriocins, small antimicrobial peptides with inhibitory activity towards pneumococcus and other related species. Here we describe an optimized bacterial overlay assay that allows for the characterization of bacteriocin activity and inhibitory spectrum, bacteriocin-specific immunity, and detection of secreted quorum sensing peptides.
Vídeos Relacionados
07:02
An Assay for Quantifying Protein-RNA Binding in Bacteria
Vídeos Relacionados
6598 Views
08:45
A Visual Assay to Monitor T6SS-mediated Bacterial Competition
Vídeos Relacionados
15657 Views
10:39
Single Cell Measurements of Vacuolar Rupture Caused by Intracellular Pathogens
Vídeos Relacionados
13515 Views
11:17
Monitoring Changes in Membrane Polarity, Membrane Integrity, and Intracellular Ion Concentrations in Streptococcus pneumoniae Using Fluorescent Dyes
Vídeos Relacionados
13663 Views
09:20
Investigating the Effects of Probiotics on Pneumococcal Colonization Using an In Vitro Adherence Assay
Vídeos Relacionados
12595 Views
12:22
Biomimetic Materials to Characterize Bacteria-host Interactions
Vídeos Relacionados
9355 Views
11:31
Establishment of a High-throughput Setup for Screening Small Molecules That Modulate c-di-GMP Signaling in Pseudomonas aeruginosa
Vídeos Relacionados
8562 Views
06:38
Use of the Soft-agar Overlay Technique to Screen for Bacterially Produced Inhibitory Compounds
Vídeos Relacionados
32490 Views
06:11
A High-throughput Shigella-specific Bactericidal Assay
Vídeos Relacionados
11745 Views
10:26
P. aeruginosa Infected 3D Co-Culture of Bronchial Epithelial Cells and Macrophages at Air-Liquid Interface for Preclinical Evaluation of Anti-Infectives
Vídeos Relacionados
10774 Views
Read Article
Cite this Article
Maricic, N., Dawid, S. Using the Overlay Assay to Qualitatively Measure Bacterial Production of and Sensitivity to Pneumococcal Bacteriocins. J. Vis. Exp. (91), e51876, doi:10.3791/51876 (2014).
Copy