Isolation and Functional Analysis of Mitochondria from Cultured Cells and Mouse Tissue

Thomas Lampl; Jo A. Crum; Taylor A. Davis; Carol Milligan; Victoria Del Gaizo Moore

doi:10.3791/52076

Journal

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Biologia

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Isolamento e Análise Funcional de mitocôndrias de células cultivadas e mouse Tissue

Isolation and Functional Analysis of Mitochondria from Cultured Cells and Mouse Tissue

JoVE Journal
Biologia

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JoVE Journal Biologia

Isolation and Functional Analysis of Mitochondria from Cultured Cells and Mouse Tissue

Isolamento e Análise Funcional de mitocôndrias de células cultivadas e mouse Tissue

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39,491 Views

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09:27 min

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March 23, 2015

DOI:

10.3791/52076-v

DOI:

10.3791/52076-v

•

09:27 min

March 23, 2015

•

39406 Views

Thomas Lampl¹, Jo A. Crum¹, Taylor A. Davis¹, Carol Milligan^2,3,4, Victoria Del Gaizo Moore¹

¹Chemistry Department,Elon University ²Department of Neurobiology and Anatomy,Wake Forest School of Medicine ³Neuroscience Graduate Program,Wake Forest School of Medicine ⁴ALS Center Translational Science Unit,Wake Forest School of Medicine

Summary

Comparison of mitochondrial membrane potential between samples yields valuable information about cellular status. Detailed steps for isolating mitochondria and assessing response to inhibitors and uncouplers using fluorescence are described. The method and utility of this protocol are illustrated by use of a cell culture and animal model of cellular stress.