The Neuromuscular Junction: Measuring Synapse Size, Fragmentation and Changes in Synaptic Protein Density Using Confocal Fluorescence Microscopy

Nigel Tse; Marco Morsch; Nazanin Ghazanfari; Louise Cole; Archunan Visvanathan; Catherine Leamey; William D. Phillips

doi:10.3791/52220

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A Junção Neuromuscular: Synapse Medir o tamanho, Mudanças de fragmentação e em Synaptic Density proteína utilizando microscopia de fluorescência confocal

JoVE Journal
Neurociência

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JoVE Journal Neurociência

The Neuromuscular Junction: Measuring Synapse Size, Fragmentation and Changes in Synaptic Protein Density Using Confocal Fluorescence Microscopy

A Junção Neuromuscular: Synapse Medir o tamanho, Mudanças de fragmentação e em Synaptic Density proteína utilizando microscopia de fluorescência confocal

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21,512 Views

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12:18 min

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December 26, 2014

DOI:

10.3791/52220-v

DOI:

10.3791/52220-v

•

12:18 min

December 26, 2014

•

26 Views

Nigel Tse¹, Marco Morsch², Nazanin Ghazanfari¹, Louise Cole³, Archunan Visvanathan¹, Catherine Leamey¹, William D. Phillips¹

¹Physiology and Bosch Institute,University of Sydney, ²Motor Neuron Disease Research Group, Australian School of Advanced Medicine,Macquarie University, ³Advanced Microscopy Facility, Bosch Institute,University of Sydney

Summary

The neuromuscular junction (NMJ) is altered in a variety of conditions that can sometimes culminate in synaptic failure. This report describes fluorescence microscope-based methods to quantify such structural changes.