Preparation and Characterization of SDF-1&#945;-Chitosan-Dextran Sulfate Nanoparticles

Andrew R. Bader; Tina Li; Weiping Wang; Daniel S. Kohane; Joseph Loscalzo; Ying-Yi Zhang

doi:10.3791/52323

Journal

/

Química

/

Preparação e Caracterização de SDF-1α-Quitosana As nanopartículas de sulfato de dextrano

Preparation and Characterization of SDF-1α-Chitosan-Dextran Sulfate Nanoparticles

JoVE Journal
Química

É necessária uma assinatura da JoVE para visualizar este conteúdo. Faça login ou comece sua avaliação gratuita.

JoVE Journal Química

Preparation and Characterization of SDF-1α-Chitosan-Dextran Sulfate Nanoparticles

Preparação e Caracterização de SDF-1α-Quitosana As nanopartículas de sulfato de dextrano

Please note that all translations are automatically generated. Click here for the English version.

12,317 Views

•

12:00 min

•

January 22, 2015

DOI:

10.3791/52323-v

DOI:

10.3791/52323-v

•

12:00 min

January 22, 2015

•

5 Views

Andrew R. Bader¹, Tina Li¹, Weiping Wang², Daniel S. Kohane², Joseph Loscalzo¹, Ying-Yi Zhang¹

¹Department of Medicine,Brigham and Women’s Hospital and Harvard Medical School, ²Laboratory for Biomaterials and Drug Delivery, Department of Anesthesiology, Division of Critical Care,Boston Children’s Hospital

Summary

The objective of this protocol is to incorporate SDF-1α, a stem cell homing factor, into dextran sulfate-chitosan nanoparticles. The resultant particles are measured for their size and zeta potential, as well as the content, activity, and in vitro release rate of SDF-1α from the nanoparticles.