EPA Method 1615. Measurement of Enterovirus and Norovirus Occurrence in Water by Culture and RT-qPCR. Part III. Virus Detection by RT-qPCR

G. Shay Fout; Jennifer L. Cashdollar; Shannon M. Griffin; Nichole E. Brinkman; Eunice A. Varughese; Sandhya U. Parshionikar

doi:10.3791/52646

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EPA Método 1615. Medição de Enterovirus e Norovirus Ocorrência na água por cultura e RT-qPCR. Parte III. Detecção de vírus por RT-qPCR

EPA Method 1615. Measurement of Enterovirus and Norovirus Occurrence in Water by Culture and RT-qPCR. Part III. Virus Detection by RT-qPCR

JoVE Journal
Ambiente

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JoVE Journal Ambiente

EPA Method 1615. Measurement of Enterovirus and Norovirus Occurrence in Water by Culture and RT-qPCR. Part III. Virus Detection by RT-qPCR

EPA Método 1615. Medição de Enterovirus e Norovirus Ocorrência na água por cultura e RT-qPCR. Parte III. Detecção de vírus por RT-qPCR

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12,693 Views

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12:32 min

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January 16, 2016

DOI:

10.3791/52646-v

DOI:

10.3791/52646-v

•

12:32 min

January 16, 2016

•

12673 Views

G. Shay Fout¹, Jennifer L. Cashdollar¹, Shannon M. Griffin¹, Nichole E. Brinkman¹, Eunice A. Varughese¹, Sandhya U. Parshionikar²

¹National Exposure Research Laboratory,U.S. Environmental Protection Agency ²Technical Services Center, Office of Ground Water and Drinking Water,U.S. Environmental Protection Agency

Summary

Here we present a procedure to quantify enterovirus and norovirus in environmental and drinking waters using reverse transcription-quantitative PCR. Mean virus recovery from groundwater with this standardized procedure from EPA Method 1615 was 20% for poliovirus and 30% for murine norovirus.