Methods to Inhibit Bacterial Pyomelanin Production and Determine the Corresponding Increase in Sensitivity to Oxidative Stress

Laura M. Ketelboeter; Sonia L. Bardy

doi:10.3791/53105

/

Métodos para inibir bacteriana Pyomelanin Produção e determinar o correspondente aumento de sensibilidade ao estresse oxidativo

Methods to Inhibit Bacterial Pyomelanin Production and Determine the Corresponding Increase in Sensitivity to Oxidative Stress

JoVE Journal
Imunologia e Infecção

É necessária uma assinatura da JoVE para visualizar este conteúdo. Faça login ou comece sua avaliação gratuita.

JoVE Journal Imunologia e Infecção

Methods to Inhibit Bacterial Pyomelanin Production and Determine the Corresponding Increase in Sensitivity to Oxidative Stress

Métodos para inibir bacteriana Pyomelanin Produção e determinar o correspondente aumento de sensibilidade ao estresse oxidativo

Please note that all translations are automatically generated. Click here for the English version.

8,134 Views

•

11:00 min

•

August 31, 2015

DOI:

10.3791/53105-v

DOI:

10.3791/53105-v

•

11:00 min

August 31, 2015

•

2 Views

Laura M. Ketelboeter¹, Sonia L. Bardy¹

¹Department of Biological Sciences,University of Wisconsin Milwaukee

Summary

Bacterial pyomelanin production results in increased resistance to oxidative stress and virulence. We report on techniques that can be used to determine inhibition of pyomelanin production and assay the resulting increase in sensitivity to oxidative stress in bacteria, as well as determine antibiotic minimum inhibitory concentration (MIC).