Analysis of Yersinia enterocolitica Effector Translocation into Host Cells Using Beta-lactamase Effector Fusions

Manuel Wolters; Bernd Zobiak; Theresa Nauth; Martin Aepfelbacher

doi:10.3791/53115

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Análises de Yersinia enterocolitica Effector translocação em células hospedeiras Usando Beta-lactamase Effector Fusions

Analysis of <em>Yersinia enterocolitica</em> Effector Translocation into Host Cells Using Beta-lactamase Effector Fusions

JoVE Journal
Imunologia e Infecção

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JoVE Journal Imunologia e Infecção

Analysis of Yersinia enterocolitica Effector Translocation into Host Cells Using Beta-lactamase Effector Fusions

Análises de Yersinia enterocolitica Effector translocação em células hospedeiras Usando Beta-lactamase Effector Fusions

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8,534 Views

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12:23 min

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October 13, 2015

DOI:

10.3791/53115-v

DOI:

10.3791/53115-v

•

12:23 min

October 13, 2015

•

8513 Views

Manuel Wolters¹, Bernd Zobiak², Theresa Nauth¹, Martin Aepfelbacher¹

¹Institute for Medical Microbiology, Virology and Hygiene,University Medical Center Hamburg-Eppendorf ²UKE Microscopy Imaging Facility,University Medical Center Hamburg-Eppendorf

Summary

Effector translocation into host cells via a type III secretion system is a common virulence strategy among gram-negative bacteria. A beta-lactamase effector fusion based assay for quantitative analysis of translocation was applied. In Yersinia infected cells, conversion of a FRET reporter by the beta-lactamase is monitored using laser scanning microscopy.