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Preparation of Mitochondrial Enriched Fractions for Metabolic Analysis in Drosophila
JoVE Journal
Biologia do Desenvolvimento
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JoVE Journal Biologia do Desenvolvimento
Preparation of Mitochondrial Enriched Fractions for Metabolic Analysis in Drosophila

Preparation of Mitochondrial Enriched Fractions for Metabolic Analysis in Drosophila

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08:22 min

September 30, 2015

DOI:

08:22 min
September 30, 2015

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Summary

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Mitochondria play central roles in the regulation of metabolism and homeostasis. Subtle changes in mitochondrial metabolism that affect organismal physiology could be difficult to detect in whole organism metabolomics studies. Here we describe an isolation method that enhances the detection of subtle metabolic shifts in Drosophila melanogaster.

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