Isolation of Mitochondria from Minimal Quantities of Mouse Skeletal Muscle for High Throughput Microplate Respiratory Measurements

Nabil E. Boutagy; Emily Pyne; George W. Rogers; Mostafa Ali; Matthew W. Hulver; Madlyn I. Frisard

doi:10.3791/53217

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Isolamento de mitocôndrias de quantidades mínimas de Rato Músculo Esquelético para medições High Throughput Microplate respiratórias

Isolation of Mitochondria from Minimal Quantities of Mouse Skeletal Muscle for High Throughput Microplate Respiratory Measurements

JoVE Journal
Biologia

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JoVE Journal Biologia

Isolation of Mitochondria from Minimal Quantities of Mouse Skeletal Muscle for High Throughput Microplate Respiratory Measurements

Isolamento de mitocôndrias de quantidades mínimas de Rato Músculo Esquelético para medições High Throughput Microplate respiratórias

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12,732 Views

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10:12 min

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November 13, 2015

DOI:

10.3791/53217-v

DOI:

10.3791/53217-v

•

10:12 min

November 13, 2015

•

7 Views

Nabil E. Boutagy^1,2, Emily Pyne¹, George W. Rogers³, Mostafa Ali¹, Matthew W. Hulver^1,2, Madlyn I. Frisard^1,2

¹The Department of Human Nutrition, Foods, and Exercise,Virginia Tech, ²The Metabolic Phenotyping Core,Virginia Tech, ³Seahorse Bioscience

Summary

Here, we present a modification of a previously reported method that allows for the isolation of high quality and purified mitochondria from smaller quantities of mouse skeletal muscle. This procedure results in highly coupled mitochondria that respire with high function during microplate based respirometirc assays.