Using Ex Vivo Upright Droplet Cultures of Whole Fetal Organs to Study Developmental Processes during Mouse Organogenesis

Sarah J. Potter; Tony DeFalco

doi:10.3791/53262

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Usando Ex Vivo Culturas Vertical Gota de toda fetais Órgãos para estudar processos de desenvolvimento durante Rato Organogenesis

Using <em>Ex Vivo</em> Upright Droplet Cultures of Whole Fetal Organs to Study Developmental Processes during Mouse Organogenesis

JoVE Journal
Biologia do Desenvolvimento

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JoVE Journal Biologia do Desenvolvimento

Using Ex Vivo Upright Droplet Cultures of Whole Fetal Organs to Study Developmental Processes during Mouse Organogenesis

Usando Ex Vivo Culturas Vertical Gota de toda fetais Órgãos para estudar processos de desenvolvimento durante Rato Organogenesis

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12,421 Views

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09:47 min

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October 21, 2015

DOI:

10.3791/53262-v

DOI:

10.3791/53262-v

•

09:47 min

October 21, 2015

•

14 Views

Sarah J. Potter¹, Tony DeFalco¹

¹Division of Reproductive Sciences,Cincinnati Children’s Hospital Medical Center

Summary

The ex vivo upright droplet culture is an alternative to current in vitro and in vivo experimental techniques. This protocol is easy to perform and requires smaller amounts of reagent, while permitting the ability to manipulate and study fetal vascularization, morphogenesis, and organogenesis.