Measuring Physiological Responses of Drosophila Sensory Neurons to Lipid Pheromones Using Live Calcium Imaging

Shruti Shankar; Meredith E.K. Calvert; Joanne Y. Yew

doi:10.3791/53392

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Medindo respostas fisiológicas de Drosophila Neurônios sensoriais para Lipid feromonas Usando Imagem de cálcio ao vivo

Measuring Physiological Responses of <em>Drosophila</em> Sensory Neurons to Lipid Pheromones Using Live Calcium Imaging

JoVE Journal
Imunologia e Infecção

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JoVE Journal Imunologia e Infecção

Measuring Physiological Responses of Drosophila Sensory Neurons to Lipid Pheromones Using Live Calcium Imaging

Medindo respostas fisiológicas de Drosophila Neurônios sensoriais para Lipid feromonas Usando Imagem de cálcio ao vivo

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8,570 Views

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07:25 min

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April 29, 2016

DOI:

10.3791/53392-v

DOI:

10.3791/53392-v

•

07:25 min

April 29, 2016

•

8535 Views

Shruti Shankar^1,2, Meredith E.K. Calvert^3,4, Joanne Y. Yew^1,2,5

¹Temasek Life Sciences Laboratory ²Department of Biological Science,National University of Singapore ³Bioimaging and Biocomputing Facility,Temasek Life Sciences Laboratory ⁴Histology and Light Microscopy Core,Gladstone Institutes ⁵Pacific Biosciences Research Center,University of Hawai’i at Mānoa

Summary

The forelegs and proboscis of Drosophila contain a rich repertoire of gustatory sensory neurons. Here, we present a method using calcium imaging to measure physiological responses from sensory neurons in the foreleg and proboscis of live flies upon exogenous application of a gustatory pheromone.