Imaging Subcellular Structures in the Living Zebrafish Embryo

Peter Engerer; Gabriela Plucinska; Rachel Thong; Laura Trov&ograve;; Dominik Paquet; Leanne Godinho

doi:10.3791/53456

Journal

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Biologia do Desenvolvimento

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Imagiologia estruturas celulares no embrião vivo Zebrafish

JoVE Journal
Biologia do Desenvolvimento

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JoVE Journal Biologia do Desenvolvimento

Imaging Subcellular Structures in the Living Zebrafish Embryo

Imagiologia estruturas celulares no embrião vivo Zebrafish

Please note that all translations are automatically generated. Click here for the English version.

11,519 Views

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11:19 min

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April 02, 2016

DOI:

10.3791/53456-v

DOI:

10.3791/53456-v

•

11:19 min

April 02, 2016

•

4 Views

Peter Engerer¹, Gabriela Plucinska^1,2, Rachel Thong^1,3, Laura Trovò¹, Dominik Paquet^4,5,6, Leanne Godinho¹

¹Institute of Neuronal Cell Biology,Technische Universität München, ²Cell Biology, Department of Biology, Faculty of Science,Utrecht University, ³Faculty of Biology,Ludwig-Maximilians-Universität-München, ⁴Adolf-Butenandt-Institute, Biochemistry,Ludwig-Maximilians-Universität-München, ⁵German Center for Neurodegenerative Diseases, ⁶Laboratory of Brain Development and Repair,The Rockefeller University

Transcrição

Summary

Imaging the dynamic behavior of organelles and other subcellular structures in vivo can shed light on their function in physiological and disease conditions. Here, we present methods for genetically tagging two organelles, centrosomes and mitochondria, and imaging their dynamics in living zebrafish embryos using wide-field and confocal microscopy.