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Construction of Modular Hydrogel Sheets for Micropatterned Macro-scaled 3D Cellular Architecture

Article DOI: 10.3791/53475-v • 10:55 min • January 11th, 2016
January 11th, 2016 •
1, 1, 1
1Department of Bio and Brain Engineering, Korea Advanced Institute of Science and Technology (KAIST)

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We describe the fabrication of micropatterned hydrogel sheets using a simple process, which can be assembled and manipulated in a freestanding form. Using these modular hydrogel sheets, a simple macro-scaled 3D cell culture system can be generated with a controlled cellular microenvironment.

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Modular Hydrogel Sheets Micropatterned Macro-scaled 3D Cellular Architecture Fabrication Manipulation Assembly 3D Cell Culture System Tissue Engineering In Vivo-like Functional Engineered Tissues Cultured Micro-environments Cost-effective Cell-based Assays Biological Studies Geometries Composition Micro-and Macro-environments In Vitro Cell-based Assays Transplantable Scaffold Photolithography Techniques Liver Lobule-like Mesh Pattern PDMS (Polydimethylsiloxane) Curing Agent Solution
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