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Designing, Packaging, and Delivery of High Titer CRISPR Retro and Lentiviruses via Stereotaxic Injection

Article DOI: 10.3791/53783-v • 11:28 min • May 23rd, 2016
May 23rd, 2016 •
1, 1, 1, 1, 1
1Department of Physiology and Neurobiology, Geisel School of Medicine at Dartmouth College

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The CRISPR/Cas9 system offers the potential to make targeted genome editing accessible and affordable to the scientific community. This protocol is intended to demonstrate how to create viruses that will knockout a gene of interest using the CRISPR/Cas9 system, and then inject them stereotaxically into the adult mouse brain.

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Designing Packaging Delivery High Titer CRISPR Retrovirus CRISPR Lentivirus Stereotaxic Injection Mouse Brain Fluorescent Protein CAS9 SgRNAs Neuroscience Neurological Disorders Neuro-developmental Disorders Gene Manipulation Genetically Engineered Animals Guide Strand Single Stranded Oligos Plasmid Creation Cell Culture Dish Incubation
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