<em>In Vivo</em> Alkaline Comet Assay and Enzyme-modified Alkaline Comet Assay for Measuring DNA Strand Breaks and Oxidative DNA Damage in Rat Liver

Wei Ding; Michelle E. Bishop; Lascelles E. Lyn-Cook; Kelly J. Davis; Mugimane G. Manjanatha

doi:10.3791/53833

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In Vivo Alkaline Ensaio Cometa e Enzyme-modificado Alkaline Comet Ensaio para medição Breaks DNA Strand e dano oxidativo ao DNA em Rat Liver

<em>In Vivo</em> Alkaline Comet Assay and Enzyme-modified Alkaline Comet Assay for Measuring DNA Strand Breaks and Oxidative DNA Damage in Rat Liver

JoVE Journal
Biologia

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JoVE Journal Biologia

In Vivo Alkaline Comet Assay and Enzyme-modified Alkaline Comet Assay for Measuring DNA Strand Breaks and Oxidative DNA Damage in Rat Liver

In Vivo Alkaline Ensaio Cometa e Enzyme-modificado Alkaline Comet Ensaio para medição Breaks DNA Strand e dano oxidativo ao DNA em Rat Liver

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15,305 Views

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10:38 min

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May 04, 2016

DOI:

10.3791/53833-v

DOI:

10.3791/53833-v

•

10:38 min

May 04, 2016

•

3 Views

Wei Ding¹, Michelle E. Bishop¹, Lascelles E. Lyn-Cook¹, Kelly J. Davis², Mugimane G. Manjanatha¹

¹Division of Genetic and Molecular Toxicology,US FDA/National Center for Toxicological Research, ²Toxicologic Pathology Associates,US FDA/National Center for Toxicological Research

Transcrição

Summary

The alkaline comet assay measures DNA strand breaks in eukaryotic cells. By adding an Endonuclease III or human 8-oxoguanine-DNA-N-glycosylase digestion step, the assay can efficiently detect oxidative DNA damage. We describe methods for using these assays to detect DNA damage in rat liver.