This manuscript describes the setup, implementation, and analysis of boldness, aggression, and shoaling in zebrafish and testing for the presence of a behavioral syndrome. A standardized approach for behavioral quantification will allow for easier comparison across studies. Modifications to this protocol are possible as each assay can be run individually.
Way, G. P., Southwell, M., McRobert, S. P. Boldness, Aggression, and Shoaling Assays for Zebrafish Behavioral Syndromes. J. Vis. Exp. (114), e54049, doi:10.3791/54049 (2016).