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Targeted RNA Sequencing Assay to Characterize Gene Expression and Genomic Alterations

Article DOI: 10.3791/54090-v • 11:52 min • August 4th, 2016
August 4th, 2016 •
1, 1, 1, 1,2
1Department of Internal Medicine, Division of Medical Oncology, Comprehensive Cancer Center, The Ohio State University, 2Department of Pharmacology, The Ohio State University

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We describe a targeted RNA sequencing-based method that includes preparation of indexed cDNA libraries, hybridization and capture with custom probes and data analysis to interrogate selected transcripts for gene expression, mutations, and gene fusions. Targeted RNAseq permits cost-effective, rapid evaluation of selected transcripts on a desktop sequencer.

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Targeted RNA Sequencing Assay Gene Expression Genomic Alterations Next Generation Sequencing Ribosomal RNA Depletion Complementary DNA Synthesis Barcoded Libraries Hybridization Target Capture Post Capture PCR Amplification Molecular Biology Gene Fusions Single Nucleotide Variations Relative Gene Expression Alternative Splicing Customizable Technique DNA Samples Cot-1 DNA Blocking Oligos Adapter Specific Blocking Oligos
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