Recording Temperature-induced Neuronal Activity through Monitoring Calcium Changes in the Olfactory Bulb of <em>Xenopus laevis</em>

Alexander Brinkmann; Camille Okom; Eugen Kludt; Detlev Schild

doi:10.3791/54108

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Gravação induzida pela temperatura actividade neuronal através de monitoramento das alterações de cálcio no bulbo olfativo de<em> Xenopus laevis</em

Recording Temperature-induced Neuronal Activity through Monitoring Calcium Changes in the Olfactory Bulb of <em>Xenopus laevis</em>

JoVE Journal
Neurociência

This content is Free Access.

JoVE Journal Neurociência

Recording Temperature-induced Neuronal Activity through Monitoring Calcium Changes in the Olfactory Bulb of Xenopus laevis

Gravação induzida pela temperatura actividade neuronal através de monitoramento das alterações de cálcio no bulbo olfativo de<em> Xenopus laevis</em

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7,280 Views

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11:08 min

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June 03, 2016

DOI:

10.3791/54108-v

DOI:

10.3791/54108-v

•

11:08 min

June 03, 2016

•

7246 Views

Alexander Brinkmann*^1,2, Camille Okom*^1,2, Eugen Kludt^1,2,4, Detlev Schild^1,2,3

¹Institute of Neurophysiology and Cellular Biophysics,Georg-August-Universität Göttingen ²Center for Nanoscale Microscopy and Molecular Physiology of the Brain,Georg-August-Universität Göttingen ³DFG Excellence Cluster 171,Georg-August-Universität Göttingen ⁴German Hearing Center Hannover

Summary

Here we describe a protocol for measuring and analyzing temperature responses in the olfactory bulb of Xenopus laevis. Olfactory receptor neurons and mitral cells are differentially stained, after which calcium changes are recorded, reflecting a sensitivity of some neural networks in the bulb to temperature drops induced at the nose.