Electrophysiological Method for Recording Intracellular Voltage Responses of Drosophila Photoreceptors and Interneurons to Light Stimuli In Vivo

Mikko Juusola; An Dau; Lei Zheng; Diana Rien

doi:10.3791/54142

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Método eletrofisiológico para respostas gravação intracelular de tensão Drosophila Fotorreceptores e Interneurônios à luz Estímulos In Vivo</em

Electrophysiological Method for Recording Intracellular Voltage Responses of <em>Drosophila</em> Photoreceptors and Interneurons to Light Stimuli <em>In Vivo</em>

JoVE Journal
Neurociência

This content is Free Access.

JoVE Journal Neurociência

Electrophysiological Method for Recording Intracellular Voltage Responses of Drosophila Photoreceptors and Interneurons to Light Stimuli In Vivo

Método eletrofisiológico para respostas gravação intracelular de tensão Drosophila Fotorreceptores e Interneurônios à luz Estímulos In Vivo</em

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19,500 Views

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11:42 min

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June 19, 2016

DOI:

10.3791/54142-v

DOI:

10.3791/54142-v

•

11:42 min

June 19, 2016

•

19496 Views

Mikko Juusola^1,2, An Dau², Lei Zheng¹, Diana Rien^1,2

¹National Key Laboratory of Cognitive Neuroscience and Learning,Beijing Normal University ²Department of Biomedical Science,The University of Sheffield

Summary

Sharp microelectrodes enable accurate electrophysiological characterization of photoreceptor and visual interneuron output in living Drosophila. Here we show how to use this method to record high-quality voltage responses of individual cells to controlled light stimulation. This method is ideal for studying neural information processing in insect compound eyes.