Aplicando Transferência de ressonância de fluorescência Energia (FRET) para examinar Eficiência Effector translocação pela<em> Burnetii Coxiella</em> Durante siRNA silenciamento
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July 06, 2016
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Investigar as interações entre patógenos bacterianos e seus hospedeiros é uma importante área de pesquisa biológica. Aqui, descrevemos as técnicas necessárias para medir efetoras translocação por Coxiella burnetii durante o silenciamento do gene siRNA usando substrato BlaM.
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Applying Fluorescence Resonance Energy Transfer (FRET) to Examine Effector Translocation Efficiency by Coxiella burnetii during siRNA Silencing
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Newton, P., Latomanski, E. A., Newton, H. J. Applying Fluorescence Resonance Energy Transfer (FRET) to Examine Effector Translocation Efficiency by Coxiella burnetii during siRNA Silencing. J. Vis. Exp. (113), e54210, doi:10.3791/54210 (2016).
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