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Filtration Isolation of Nucleic Acids: A Simple and Rapid DNA Extraction Method
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Biologia
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JoVE Journal Biologia
Filtration Isolation of Nucleic Acids: A Simple and Rapid DNA Extraction Method

Filtration Isolation of Nucleic Acids: A Simple and Rapid DNA Extraction Method

DOI:
10.3791/54289-v

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07:56 min

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August 06, 2016

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, , ,
1Center for Innovation in Global Health Technologies (CIGHT), Department of Biomedical Engineering,Northwestern University, 2Pritzker School of Medicine,The University of Chicago

Capítulos

  • 00:05Título
  • 01:08Preparation of FINA Sample Preparation Module
  • 02:27Preparation of qPCR Tube
  • 03:12Preparation of Contrived Specimen
  • 04:00FINA Extraction
  • 05:57Results: FINA Enables Efficient Detection of Proviral HIV DNA from Whole Blood
  • 07:20Conclusion

Summary

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We describe here a simple and rapid paper-based DNA extraction method of HIV proviral DNA from whole blood detected by quantitative PCR. This protocol can be extended for use in detecting other genetic markers or using alternative amplification methods.

Tags

Filtration IsolationNucleic Acid ExtractionDNA ExtractionRapid DNA ExtractionPaper-based DNA ExtractionFINA MethodPCR AmplificationHIV Proviral DNAQuantitative PCRSample Preparation ModuleBlotting PadParaffin FilmGlass Blood Separator MembraneDouble-coated Polyester Diagnostic TapePCR Tube

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