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Developmental Biology

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JoVE Journal Developmental Biology
Affinity Labeling Detection of Endogenous Receptors from Zebrafish Embryos
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Affinity Labeling Detection of Endogenous Receptors from Zebrafish Embryos

Article DOI: 10.3791/54405-v • 08:39 min • August 31st, 2016
August 31st, 2016 •
1, 1, 1
1Cell and Developmental Biology, Instituto de Fisiología Celular, Universidad Nacional Autónoma de México

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A novel technique for the detection of low abundance endogenous receptors present in zebrafish embryos is described. We have named it AFLIP because it consists of affinity labeling of the receptor by its ligand linked to immunoprecipitation.

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Affinity Labeling Detection Endogenous Receptors Zebrafish Embryos Low Abundance Growth Factor Receptors Radioactive Ligand Receptor Expression Levels Biochemical Properties AFLIP Developmental Biology Petri Dishes Fish Water Dechorionate Embryos Pronase Protease 1.5-milliliter Microfuge Tube 1X PBS Deyolking Buffer Yolk Release
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