Formação Light-mediada e padronização de hidrogéis para aplicações de cultura celular
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10:45 min
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September 29, 2016
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We describe a sequential process for light-mediated formation and subsequent biochemical patterning of synthetic hydrogel matrices for three-dimensional cell culture applications. The construction and modification of hydrogels with cytocompatible photoclick chemistry is demonstrated. Additionally, facile techniques to quantify and observe patterns and determine cell viability within these hydrogels are presented.
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Sawicki, L. A., Kloxin, A. M. Light-mediated Formation and Patterning of Hydrogels for Cell Culture Applications. J. Vis. Exp. (115), e54462, doi:10.3791/54462 (2016).
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