Measurement of In Vitro Integration Activity of HIV-1 Preintegration Complexes

Muthukumar Balasubramaniam; Benem Davids; Amma B. Addai; Jui Pandhare; Chandravanu Dash

doi:10.3791/54581

Journal

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Imunologia e Infecção

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Medição de In Vitro Actividade integração do HIV-1 de pré-integração Complexos

Measurement of <em>In Vitro</em> Integration Activity of HIV-1 Preintegration Complexes

JoVE Journal
Imunologia e Infecção

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JoVE Journal Imunologia e Infecção

Measurement of In Vitro Integration Activity of HIV-1 Preintegration Complexes

Medição de In Vitro Actividade integração do HIV-1 de pré-integração Complexos

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7,439 Views

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10:34 min

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February 22, 2017

DOI:

10.3791/54581-v

DOI:

10.3791/54581-v

•

10:34 min

February 22, 2017

•

1 Views

Muthukumar Balasubramaniam^1,2, Benem Davids^1,2, Amma B. Addai^1,2, Jui Pandhare^1,3,4, Chandravanu Dash^1,2,5

¹Center for AIDS Health Disparities Research,Meharry Medical College, ²Department of Biochemistry and Cancer Biology,Meharry Medical College, ³School of Graduate Studies and Research,Meharry Medical College, ⁴Department of Microbiology and Immunology,Meharry Medical College, ⁵Tennessee Center for AIDS Research (TN-CFAR),Meharry Medical College

Transcrição

Summary

This report describes an in vitro assay for measuring the human immunodeficiency virus type 1 (HIV-1) preintegration complex (PIC)-associated integration activity in the cytoplasmic extracts of acutely infected cells. The integration of PIC-associated HIV-1 DNA into heterologous target DNA is quantified by using a nested real-time polymerase chain reaction strategy.