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JoVE Journal Biochemistry
Utilizing the Ethylene-releasing Compound, 2-Chloroethylphosphonic Acid, as a Tool to Study Ethylene Response in Bacteria
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Utilizing the Ethylene-releasing Compound, 2-Chloroethylphosphonic Acid, as a Tool to Study Ethylene Response in Bacteria

Article DOI: 10.3791/54682-v • 09:05 min • November 10th, 2016
November 10th, 2016 •
1, 1, 1
1Molecular Microbial Biochemistry Laboratory, Faculty of Science (Applied Bioscience Program), University of Ontario Institute of Technology

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The protocols outlined herein facilitate the convenient investigation of bacterial ethylene responses by utilizing 2-chloroethylphosphonic acid (CEPA). Ethylene is produced in situ through the decomposition of CEPA in an aqueous bacterial growth medium, circumventing the requirement for pure ethylene gas.

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Ethylene-releasing Compound 2-Chloroethylphosphonic Acid Ethylene Response Bacteria Plant-microbe Interactions Bacterial Adhesion Colonization Of Plants Specialized Equipment Gaseous Ethylene Bacterial Cellulose Production Plant-associated Microorganisms In Vitro Study Ethylene-mediated Processes Plant Colonization Growth Medium Arabidopsis Seedlings Bacterial Growth Medium CEPA Decomposition Plate Quadrants Agar Solidification
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