Detection of Trypanosoma brucei Variant Surface Glycoprotein Switching by Magnetic Activated Cell Sorting and Flow Cytometry

Danae Schulz; Monica R. Mugnier; Catherine E. Boothroyd; F. Nina Papavasiliou

doi:10.3791/54715

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detecção de Trypanosoma brucei Switching Variant glicoproteína de superfície por Magnetic celular ativado Seleção e citometria de fluxo

JoVE Journal
Imunologia e Infecção

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JoVE Journal Imunologia e Infecção

Detection of Trypanosoma brucei Variant Surface Glycoprotein Switching by Magnetic Activated Cell Sorting and Flow Cytometry

detecção de Trypanosoma brucei Switching Variant glicoproteína de superfície por Magnetic celular ativado Seleção e citometria de fluxo

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8,652 Views

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09:45 min

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October 19, 2016

DOI:

10.3791/54715-v

DOI:

10.3791/54715-v

•

09:45 min

October 19, 2016

•

1 Views

Danae Schulz^1,2, Monica R. Mugnier¹, Catherine E. Boothroyd^1,3, F. Nina Papavasiliou¹

¹Laboratory of Lymphocyte Biology,Rockefeller University, ²Department of Biology,Harvey Mudd College, ³Masters School

Transcrição

Summary

African trypanosomes grown in vitro undergo antigenic variation at a low rate, such that populations are made up of parasites expressing a dominant variant surface glycoprotein (VSG) type and a small population of "switched" variants. This protocol describes a fast method for detecting and quantifying these populations.