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Confirmation of Myocardial Ischemia and Reperfusion Injury in Mice Using Surface Pad Electrocardiography
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Confirmation of Myocardial Ischemia and Reperfusion Injury in Mice Using Surface Pad Electrocardiography

Article DOI: 10.3791/54814-v • 09:23 min • November 24th, 2016
November 24th, 2016 •
1, 1,2
1Department of Biomedical Sciences, James H. Quillen College of Medicine, East Tennessee State University, 2James H. Quillen Veterans Affairs Medical Center

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During murine myocardial ischemia/reperfusion surgery, correct placement of the occluding ligature is typically confirmed by visible observation of myocardial pallor. Herein, a method of electrocardiographically confirming ischemia and reperfusion, to supplement observed myocardial pallor, is demonstrated in male C57Bl/6 mice.

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Electrocardiography Myocardial Ischemia Reperfusion Injury Mice Surface Pad Procedure Consistency Reproducibility Ischemia Reperfusion Surgery Technique Success Assessment ECG Pad Electrolyte Gel Metallic Electrodes Anesthetized Mouse Tape Machine Configuration Real-time Visualization Waveform Resolution R Wave Peak Q Wave Trough Baseline Waveform Ischemia Reperfusion Procedure
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