Chondrogenic Differentiation Induction of Adipose-derived Stem Cells by Centrifugal Gravity

Yeonsue Jang; Hyerin Jung; Ji Hyeon Ju

doi:10.3791/54934

Condrogênica Diferenciação indução de células-tronco derivadas de tecido adiposo por gravidade ce...

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JoVE Journal Developmental Biology

Chondrogenic Differentiation Induction of Adipose-derived Stem Cells by Centrifugal Gravity

Condrogênica Diferenciação indução de células-tronco derivadas de tecido adiposo por gravidade centrífugas

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08:30 min

February 24, 2017

DOI: 10.3791/54934-v

Yeonsue Jang¹, Hyerin Jung¹, Ji Hyeon Ju¹

¹CiSTEM Laboratory, Convergent Research Consortium for Immunologic Disease, Division of Rheumatology, Seoul St. Mary’s Hospital, College of Medicine,The Catholic University of Korea

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Overview

This article presents a protocol for inducing chondrogenic differentiation of adipose-derived stem cells (ASCs) using centrifugal gravity (CG). The method highlights the potential of mechanical stress in cartilage repair by promoting the upregulation of SOX9, leading to the development of chondrogenic phenotypes.

Key Study Components

Area of Science

Stem Cell Biology
Cartilage Repair
Mechanical Stress Effects

Background

Chondrogenic differentiation is crucial for cartilage repair.
Adipose-derived stem cells (ASCs) are a promising source for regenerative therapies.
Mechanical forces can influence stem cell differentiation.
SOX9 is a key transcription factor in chondrogenesis.

Purpose of Study

To develop a method for inducing chondrogenic differentiation of ASCs.
To explore the effects of centrifugal gravity on stem cell differentiation.
To provide a cytokine-free approach for cartilage repair.

Methods Used

Harvesting ASCs from culture dishes.
Applying centrifugal gravity to induce differentiation.
Assessing the expression of chondrogenic markers.
Demonstrating the procedure by laboratory personnel.

Main Results

Successful induction of chondrogenic differentiation in ASCs.
Upregulation of SOX9 observed following treatment.
Development of chondrogenic phenotypes confirmed.
Potential applications in other differentiation pathways noted.

Conclusions

Centrifugal gravity is an effective method for inducing chondrogenic differentiation.
This technique may enhance cartilage repair strategies.
Further research could expand its application to other stem cell differentiation processes.

Frequently Asked Questions

What is the main goal of this study?

The main goal is to induce chondrogenic differentiation of adipose-derived stem cells for cartilage repair.

How does centrifugal gravity influence stem cells?

Centrifugal gravity induces mechanical stress that promotes differentiation into chondrogenic phenotypes.

What are the advantages of this method?

It allows for differentiation without the use of cytokines, simplifying the process.

Who demonstrated the procedure?

Yeri Alice Rim and Yoojun Nam from the laboratory demonstrated the procedure.

Can this method be applied to other types of differentiation?

Yes, it can also be applied to osteogenic and fibrogenic differentiation.

What is SOX9's role in this process?

SOX9 is a key transcription factor that regulates chondrogenic differentiation.

O estresse mecânico pode induzir a diferenciação condrogênica de células-tronco, fornecendo uma abordagem terapêutica potencial para o reparo da cartilagem prejudicada. Apresentamos um protocolo para induzir a diferenciação condrogênica de células-tronco derivadas do tecido adiposo (ASCs) usando gravidade centrífuga (CG). A regulação positiva de SOX9 induzida por CG resulta no desenvolvimento de fenótipos condrogênicos.

O objetivo geral deste procedimento é induzir a diferenciação condrogênica de ASCs para reparo defeituoso da cartilagem. Este método é induzir a diferenciação pré-condrogênica de ASC usando a força da gravidade centrífuga. Embora este método possa fornecer informações sobre a diferenciação condrogênica de ASCs, ele também pode ser aplicado a outros sistemas, como diferenciação osteogênica e fibrogênica.

A principal vantagem dessa técnica é que a diferenciação condrogênica pode ser facilmente induzida a partir de células-tronco sem tratar nenhuma citocina. Demonstrando o procedimento estarão Yeri Alice Rim e Yoojun Nam do meu laboratório. Comece colhendo células-tronco derivadas de tecido adiposo 80% confluentes de uma placa de cultura de 100 mililitros, descartando o meio e lavando com cinco mililitros de PBS.

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