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JoVE Journal Neuroscience
Derivation of Leptomeninges Explant Cultures from Postmortem Human Brain Donors
Automated Interactive Video Playback for Studies of Animal Communication
Journal (Neuroscience)
Automated Interactive Video Playback for Studies of Animal Communication
Combining Lipophilic dye, in situ Hybridization, Immunohistochemistry, and Histology
Journal (Neuroscience)
Combining Lipophilic dye, in situ Hybridization, Immunohistochemistry, and Histology
 

Derivation of Leptomeninges Explant Cultures from Postmortem Human Brain Donors

Article DOI: 10.3791/55045-v • 05:18 min • January 21st, 2017
January 21st, 2017 •
*1, *1, 1, 1, 1
1Parkinson’s Institute and Clinical Center
* These authors contributed equally

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The leptomeninges explant culture protocol from human postmortem brain is a technically robust and simple way to derive fibronectin-positive meningeal fibroblasts within 6-8 weeks and cryopreserve approximately 20-30 million cells.

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Leptomeninges Explant Cultures Postmortem Human Brain Donors Parkinson's Disease Fibroblast Cell-based Assays Lewy Body Pathology Tissue Culture Hood Phosphate-buffered Saline Cutting Dish Sterile Forceps Blood Removal
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