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JoVE Journal Developmental Biology
Production and Administration of Therapeutic Mesenchymal Stem/Stromal Cell (MSC) Spheroids Primed in 3-D Cultures Under Xeno-free Conditions
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Production and Administration of Therapeutic Mesenchymal Stem/Stromal Cell (MSC) Spheroids Primed in 3-D Cultures Under Xeno-free Conditions

Article DOI: 10.3791/55126-v • 11:05 min • March 18th, 2017
March 18th, 2017 •
1, 2, 3, 4
1Department of Biology, University of Mary Hardin-Baylor, 2Department of Pediatrics, University of Texas Medical Branch, 3Multi-Organ Support Technology Task Area, U.S. Army Institute of Surgical Research, 4Internal Medicine, Texas A&M University Health Science Center

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The therapeutic potential of mesenchymal stem/stromal cells (MSCs) is well-documented, however the best method of preparing the cells for patients remains controversial. Herein, we communicate protocols to efficiently generate and administer therapeutic spherical aggregates or 'spheroids' of MSCs primed under xeno-free conditions for experimental and clinical applications.

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Therapeutic Mesenchymal Stem/stromal Cell MSC Spheroids 3-D Cultures Xeno-free Conditions Cell Retention Cell Survival Cell Functionality Tissue Injury Immunogenic Animal Products FBS Clinical Applications Pre-clinical Data Tissue Repair Media Formulations Josh Beaver Bret Clough Seeding Incubating Trips EDTA Harvest Centrifugation
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