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Preparation of Horizontal Slices of Adult Mouse Retina for Electrophysiological Studies
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Preparation of Horizontal Slices of Adult Mouse Retina for Electrophysiological Studies

Article DOI: 10.3791/55173-v • 07:02 min • January 27th, 2017
January 27th, 2017 •
1, 1
1Department of Biology, Friedrich-Alexander-University (FAU) Erlangen-Nürnberg

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We developed a horizontal slice preparation of the adult mammalian retina with the plane of sectioning parallel to the retinal surface. Dendritic fields of nonradially oriented retinal neurons were not truncated, allowing the study of signal processing with patch-clamp and imaging techniques.

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Horizontal Slice Preparation Adult Mouse Retina Electrophysiological Studies Morphological Integrity Physiological Integrity Horizontally Oriented Neurons Functional Role Synaptic Circuits Visual Information Processing Ribbon Synapses Horizontal Cells Amacrine Cells Electronic Recordings Procedure Andrea Nerz Technician Laboratory Remove Eyeball Curved Forceps Optic Nerve Spring Scissor Transfer Eyeball Petri Dish AMS Medium Stereo Microscope Magnification Visual Control Puncture Cornea Sclera Transition Damage Retina
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