Non-invasive <em>In Vivo</em> Fluorescence Optical Imaging of Inflammatory MMP Activity Using an Activatable Fluorescent Imaging Agent

Johannes Schwenck; Florian C. Maier; Manfred Kneilling; Stefan Wiehr; Kerstin Fuchs

doi:10.3791/55180

Non-invasive In Vivo Fluorescência de imagem óptico de Inflamatória MMP Atividade Usando...

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Immunology and Infection

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JoVE Journal Immunology and Infection

Non-invasive In Vivo Fluorescence Optical Imaging of Inflammatory MMP Activity Using an Activatable Fluorescent Imaging Agent

Non-invasive In Vivo Fluorescência de imagem óptico de Inflamatória MMP Atividade Usando um Agente de Imaging Activatable fluorescente

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06:46 min

May 8, 2017

DOI: 10.3791/55180-v

Johannes Schwenck^1,2, Florian C. Maier¹, Manfred Kneilling^1,3, Stefan Wiehr¹, Kerstin Fuchs¹

¹Werner Siemens Imaging Center, Department of Preclinical Imaging and Radiopharmacy,Eberhard Karls University of Tübingen, ²Department of Nuclear Medicine,Eberhard Karls University of Tübingen, ³Department of Dermatology,Eberhard Karls University of Tübingen

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Overview

This paper explains the application of fluorescent imaging using an activatable optical imaging probe to visualize the in vivo activity of key matrix metalloproteinases in two different experimental models of inflammation. This method can help answer key questions about underlying biological mechanisms in inflammatory diseases.

Key Study Components

Area of Science

Neuroscience
Biology
Imaging Techniques

Background

Matrix metalloproteinases (MMPs) play a crucial role in inflammation.
Understanding MMP activity can provide insights into inflammatory diseases.
Fluorescent imaging techniques are valuable for real-time observation.
Activatable probes enhance the specificity of imaging in biological systems.

Purpose of Study

To visualize the in vivo activity of MMPs in inflammation models.
To assess the effectiveness of optical imaging techniques.
To explore the biological mechanisms underlying inflammatory responses.

Methods Used

Use of activatable optical imaging probes.
Experimental models of inflammation.
Fluorescent imaging techniques for visualization.
Assessment of MMP activity in vivo.

Main Results

Successful visualization of MMP activity in live models.
Demonstrated the feasibility of using fluorescent probes.
Provided insights into the dynamics of inflammation.
Highlighted the advantages of the imaging technique.

Conclusions

The method is fast, cost-effective, and easy to learn.
A variety of probes are available for different biological processes.
This approach can advance the understanding of inflammatory diseases.

Frequently Asked Questions

What are matrix metalloproteinases?

Matrix metalloproteinases (MMPs) are enzymes that degrade various components of the extracellular matrix and play a significant role in tissue remodeling and inflammation.

How does fluorescent imaging work?

Fluorescent imaging involves using specific probes that emit light when excited, allowing researchers to visualize biological processes in real-time.

What are the advantages of using activatable probes?

Activatable probes provide enhanced specificity and sensitivity, allowing for better visualization of target molecules in complex biological environments.

Can this method be applied to other diseases?

Yes, the technique can potentially be adapted to study various diseases where MMP activity is relevant, including cancer and cardiovascular diseases.

Is this technique suitable for all types of imaging?

While effective for certain applications, the suitability of this technique depends on the specific biological context and the probes used.

Este documento explica a aplicação de imagem fluorescente usando uma sonda de imagiologia óptica activável para visualizar a actividade in vivo de metaloproteinases de matriz chave em dois modelos experimentais diferentes de inflamação.

O objetivo geral deste procedimento é usar imagens ópticas para visualizar uma sonda ativável para avaliar a atividade in vivo de metaloproteases de matriz chave, ou MMPs, em modelos experimentais de inflamação. Este método pode ajudar a responder a perguntas-chave sobre o mecanismo biológico subjacente em doenças inflamatórias. As principais vantagens dessa técnica são que ela é rápida, barata e fácil de aprender.

Além disso, uma grande variedade de sondas para direcionar diferentes processos biológicos está disponível. Para a indução da artrite reumatóide, primeiro use um micrômetro para medir todos os diâmetros do tornozelo de cada animal. Em seguida, levante o primeiro camundongo suavemente pela cauda e injete intraperitonealmente 200 microlitros de soro de camundongo KBN diluído contendo anticorpos contra glicose, seis fosfato isomerase ou GPI.

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