Biosensing Motor Neuron Membrane Potential in Live Zebrafish Embryos

Lorena Benedetti; Anna Ghilardi; Laura Prosperi; Maura Francolini; Luca Del Giacco

doi:10.3791/55297

Journal

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Biologia do Desenvolvimento

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Potencial de membrana de neurônio motor biossensível em embriões vivos de peixe-zebra

Biosensing Motor Neuron Membrane Potential in Live Zebrafish Embryos

JoVE Journal
Biologia do Desenvolvimento

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JoVE Journal Biologia do Desenvolvimento

Biosensing Motor Neuron Membrane Potential in Live Zebrafish Embryos

Potencial de membrana de neurônio motor biossensível em embriões vivos de peixe-zebra

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6,583 Views

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10:18 min

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June 26, 2017

DOI:

10.3791/55297-v

DOI:

10.3791/55297-v

•

10:18 min

June 26, 2017

•

1 Views

Lorena Benedetti^1,2,3, Anna Ghilardi⁴, Laura Prosperi⁴, Maura Francolini*¹, Luca Del Giacco*⁴

¹Department of Medical Biotechnology and Translational Medicine,Università degli Studi di Milano, ²Department of Neuroscience; Department of Cell Biology,Howard Hughes Medical Institute, Yale University School of Medicine, ³Program in Cellular Neuroscience, Neurodegeneration and Repair,Yale University School of Medicine, ⁴Department of BioSciences,Università degli Studi di Milano

Transcrição

Summary

Protocols described here allow for the study of the electrical properties of excitable cells in the most non-invasive physiological conditions by employing zebrafish embryos in an in vivo system together with a fluorescence resonance energy transfer (FRET)-based genetically encoded voltage indicator (GEVI) selectively expressed in the cell type of interest.