Um ensaio de HS-MRM para a quantificação de proteínas de célula hospedeira em biofármacos de proteínas por espectrometria de massa líquida cromatografia iônica mobilidade QTOF
10,070 Views
•
11:09 min
•
April 17, 2018
DOI:
Summary
Automatically generated
Aqui, descrevemos um ensaio cromatográfico, juntamente com a separação de mobilidade do íon de precursores de peptídeo seguido de alta resolução (~ 30.000) MS-detecção de fragmentos de peptídeo para a quantificação dos padrões de peptídeo cravado em um anticorpo monoclonal digerir.
Explore More Videos
Vídeos Relacionados
10:37
Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
Vídeos Relacionados
11911 Views
08:53
Quantitative and Qualitative Method for Sphingomyelin by LC-MS Using Two Stable Isotopically Labeled Sphingomyelin Species
Vídeos Relacionados
9768 Views
N/A
Carrier-assisted One-pot Sample Preparation for Targeted Proteomics Analysis of Small Numbers of Human Cells
Vídeos Relacionados
2112 Views
09:40
A Mass Spectrometry-Based Proteomics Approach for Global and High-Confidence Protein R-Methylation Analysis
Vídeos Relacionados
2279 Views
05:52
Histone Modification Screening using Liquid Chromatography, Trapped Ion Mobility Spectrometry, and Time-Of-Flight Mass Spectrometry
Vídeos Relacionados
1031 Views
09:45
Host Cell Protein Analysis using Enrichment Beads Coupled with Limited Digestion
Vídeos Relacionados
2173 Views
11:09
An HS-MRM Assay for the Quantification of Host-cell Proteins in Protein Biopharmaceuticals by Liquid Chromatography Ion Mobility QTOF Mass Spectrometry
Vídeos Relacionados
10.1K Views
05:52
Histone Modification Screening using Liquid Chromatography, Trapped Ion Mobility Spectrometry, and Time-Of-Flight Mass Spectrometry
Vídeos Relacionados
1.0K Views
08:56
Detection of Regulated Ergot Alkaloids in Food Matrices by Liquid Chromatography-Trapped Ion Mobility Spectrometry-Time-of-Flight Mass Spectrometry
Vídeos Relacionados
581 Views
08:06
Absolute Quantification of Cell-Free Protein Synthesis Metabolism by Reversed-Phase Liquid Chromatography-Mass Spectrometry
Vídeos Relacionados
9.2K Views
Read Article
Cite this Article
Doneanu, C., Fang, J., Alelyunas, Y., Yu, Y. Q., Wrona, M., Chen, W. An HS-MRM Assay for the Quantification of Host-cell Proteins in Protein Biopharmaceuticals by Liquid Chromatography Ion Mobility QTOF Mass Spectrometry. J. Vis. Exp. (134), e55325, doi:10.3791/55325 (2018).
Copy